Density Gradient Media

Excellence in Separations
 

  • Lymphoprep
  • Lymphoprep Tube
  • OptiPrep
  • Polymorphprep
  • Nycodenz
  • Polysucrose 400

OptiPrep

The optimum density gradient medium

OptiPrep is a sterile and endotoxin tested solution of 60 % iodixanol in water with a density of 1.320 g/ml. Iodixanol was developed as an X-ray contrast medium an has therefore been subjected to rigorous clinical testing. It is non-ionic, non-toxic to cells and metabolically inert. Iodixanol solutions can be made isoosmotic at all useful densities, this solutions have low viscosity and osmolality.
The high density of OptiPrep facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient or through a simple density barrier.

Improved resolution of cell organelles. Low viscosity, isoosmotic gradients provide rapid and efficient separation of the major organelles in preformed gradients.

OptiPrep is the ideal solution for virus purification.

Virus purified using OptiPrep gradients shows infectivity: particle number ratios at least 100x those from CsCl gradients.

Each batch of OptiPrep is checked on the level of endotoxins using a specific LAL test. Our goal is to produce batches with an endotoxin level lower or equal to 0.13 IU/ml.

For every batch produced a Certificate of Analysis showing the actual values of density and endotoxin levels is made available at „diagnostic.serumwerk.com“. We also claim sterility according to Ph.Eur.

OptiPrep is manufactured, packed and released in compliance with GMP and ISO13485.

Applications

Isolation of:

  • Mammalian and non-mammalian cells
  • Subcellular organelles
  • Plasma membranes and domains
  • Membrane vesicles and cytosol
  • Organelles from non-mammalian sources
  • Viruses
  • Plasma lipoproteins
  • Proteins and protein complexes
  • Plasmid DNA
  • Ribonucleoproteins

analysis of:

  • membrane trafficking and cell
    signalling

  • endocytosis and exocytosis
OptiPrep is a ready-made, sterile and endotoxin tested
solution with the following specifications:
Iodixanol 60% (w/v)
Density 1.320 ± 0.001 g/ml
Endotoxins < 1.0 IU/ml
OptiPrep is supplied as
a sterile solution in the following package sizes:
Prod. No. 1893 1 x 250 ml