Density Gradient Media
Excellence in Separations
- Lymphoprep
- Lymphoprep Tube
- OptiPrep
- Polymorphprep
- Nycodenz
- Polysucrose 400
Density Gradient Media – Downloads
Cell Applications Serumwerk
- C01 – Preparation of density gradient solutions
- C02 – Preparation of gradients for cells
- C03 – Mononuclear cells, lymphocytes, monocytes and polymorphonuclear leukocytes from blood: a methodological review
- C04 – Isolation of mononuclear cells from human blood by sedimentation on to a density barrier
- C05 – Isolation of human peripheral blood mononuclear cells by flotation (iodixanol mixer technique)
- C06 – Isolation of human peripheral blood mononuclear cells by flotation (low density iodixanol density barrier)
- C07 – Isolation of rat blood mononuclear cells by flotation (iodixanol mixer technique)
- C08 – Isolation of mononuclear cells from mouse blood by flotation (iodixanol mixer technique)
- C09 – Isolation of ruminant and equine peripheral blood mononuclear cells in iodixanol gradients
- C10 – Separation of monocytes from a leukocyte-rich plasma by flotation through a discontinuous iodixanol gradient
- C11 – Isolation of a monocyte-rich fraction from whole human blood by iodixanol barrier flotation
- C12 – Isolation of human polymorphonuclear leukocytes (granulocytes) from a leukocyte rich plasma in a discontinuous iodixanol gradient
- C13 – Isolation (removal) of human platelets (thrombocytes) on an iodixanol barrier
- C14 – Removal of non-viable cells from a cell suspension
- C15 – Fractionation of a mixed population of cells
- C16 – Purification of Islets of Langerhans from porcine, primate and rodent pancreas in a discontinuous iodixanol gradient
- C17 – Purification and processing of animal (non-human) and avian spermatozoa
- C18 – Purification of viable human spermatozoa in iodixanol or Nycodenz® gradients
- C19 – Purification of intact plant protoplasts by density gradient flotation
- C20 – Purification of leukocyte fractions from rodent/rabbit peritoneal exudates
- C21 – Dendritic cells from tissues by flotation through a low-density barrier
- C22 – Isolation of dendritic cells from tissues by a mixer technique
- C23 – Isolation of a mouse motoneuron enriched fraction from spinal cord on a density barrier
- C24 – Isolation of a progenitor cell-enriched fraction
- C25 – Hepatic non-parenchymal (stellate, Kupffer and endothelial) cells – a short methodological survey
- C26 – Purification of hepatic non-parenchymal cells on a density barrier
- C27 – Preparation of stellate cells from liver and pancreas
- C28 – Enrichment of hepatic Kupffer cells in a discontinuous gradient
- C29 – Isolation of cells from pulmonary tissue Endothelial from epithelial cells, type I epithelial cells and macrophages
- C30 – Isolation of rat alveolar type II pneumocytes, lymphoid and myeloid cells
- C31 – Purification of protozoa
- C32 – Purification of malarial parasites (Plasmodium falciparum, Plasmodium berghei, Plasmodium vivax, and Plasmodium yoelii)
- C33 – Purification of Toxoplasma gondii from cell cultures (Part A) Separation of sporocysts and oocyst walls (Part B)
- C34 – Fractionation and analysis of normal erythrocytes, sickle cells and reticulocytes; removal of erythrocytes from blood and bone marrow
- C35 – Isolation of neuroglia, inflammatory and glial cells from neural tissue
- C36 – Isolation of brain motoneurons
- C37 – Isolation of renal cells: (1) Proximal tubule cells and (2) Interstitial cells and thin loop of Henlé cells
- C38 – (I) Maintenance of cells in suspension for chemical/physical measurements (II) Microfluidic cell encapsulation/cell sorting
- C39 – Purification of bacteria from soil, stream-water, clinical specimens and food
- C40 – Isolation of mononuclear cells from tissues
- C41 – Isolation of dendritic cells from tissues by sedimentation on to a density barrier
- C42 – Purification of macrophages and foam cells
- C43 – Isolation of mononuclear cells from rat, mouse and rabbit blood on a density barrier
- C44 – Isolation of polymorphonuclear leukocytes (granulocytes) from rat, mouse, guinea pig and rabbit blood
- C45 – Isolation of peripheral blood mononuclear cells from non-human primates
- C46 – Isolation of monocytes from a human leukocyte-rich plasma on a density barrier
- C47 – Purification of enterochromaffin-like (ECL) cells from gastric mucosa
- C48 – Fractionation of parietal and chief cells from the gastric mucosa
- C49 – Purification of bacteria and bacterial minicells from cultured cells
- C50 – Fractionation of coelomocyte cell populations from sea urchins
- C51 – Analysis of gradients
- Cell-index-Serumwerk
Cell references Serumwerk
- RC01 – Mononuclear cells, monocytes and polymorphonuclear leukocytes
- RC02 – Purification of platelets from whole blood/removal from blood leukocyte preparations
- RC03 – Viable/non-viable cell separation
- RC04 – Purification of pancreatic islets
- RC05 – Dendritic cells from blood and tissues
- RC06 – Isolation of cells from brain and spinal cord
- RC07 – Hepatic and pancreatic stellate cells
- RC08 – Hepatic non-parenchymal cells: Kupffer cells, sinusoidal endothelial cells (and other liver cell types)
- RC09 – Pulmonary cells
Instruction for Use
Material Data Safety Sheet
Macromolecules Applications Serumwerk
- M01 – Preparation of gradient solutions (for macromolecules and macromolecular complexes)
- M02 – Preparation of discontinuous and continuous gradients (for macromolecules and macromolecular complexes)
- M03 – Self-generated gradients (macromolecules/macromolecular complexes)
- M04 – Harvesting gradients
- M05 – Analysis of gradients
- M06 – Isolation of plasmid DNA in self-generated gradients of iodixanol using DAPI as a fluorescent marker
- M07 – Analysis of mammalian and non-mammalian HDL, LDL and VLDL
- M08 – Fractionation of plasma lipoprotein sub-classes
- M09 – Analysis of protein complex formation, microtubules and cytoskeleton
- M10 – Separation of proteoliposomes from proteins and liposome-encapsulated macromolecules from liposomes
- M11 – Analysis of protein size in pre-formed gradients
- M12 – Analysis of proteins in small volume self-generated gradients
- M13 – Fractionation of nucleic acids and nucleic acid-protein complexes
- Macromolecules-indexes-Serumwerk
Macromolecules References Serumwerk
Subcellular Applications Serumwerk
- S01 – Preparation of gradient solutions (mammalian)
- S02 – Preparation of gradient solutions (non-mammalian)
- S03 – Preparation of discontinuous and continuous gradients
- S04 – Preparation of self-generated gradients
- S05 – Homogenization of mammalian tissues
- S06 – Homogenization of mammalian cells
- S07 – Differential centrifugation of homogenates
- S08 – Harvesting gradients
- S09 – Analysis of gradients
- S10a – Purification of nuclei from tissues and cells in iodixanol gradients – a methodological review
- S10 – Isolation of nuclei from animal and plant sources and cultured cells
- S11 – Purification of mammalian peroxisomes in a continuous gradient
- S12 – Purification of mammalian peroxisomes on a discontinuous gradient or density barrier
- S13 – Purification of mammalian peroxisomes in a self-generated gradient
- S14a – Purification and analysis of mammalian mitochondrial fractions
- S14 – Purification of mammalian mitochondria in a discontinuous gradient
- S15 – Light mitochondrial fraction analysis (mitochondria, lysosomes and peroxisomes) in continuous gradients
- S16 – Light mitochondrial fraction analysis (mitochondria, lysosomes and peroxisomes) in self-generated gradients
- S17 – Purification of yeast spheroplast mitochondria
- S18 – Fractionation of rough and smooth endoplasmic reticulum in self-generated gradients
- S19 – Fractionation of rough and smooth endoplasmic reticulum and subfractionation of ER from cultured mammalian cells
- S20 – Fractionation of smooth and rough endoplasmic reticulum and separation from Golgi (and other organelles)
- S21 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC, TGN from mammalian cells and tissues in continuous iodixanol gradients (1.5-3 h spin)
- S22 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from mammalian cells and tissues in continuous gradients (16-18 h spin)
- S23 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from mammalian cells and tissues by flotation in discontinuous gradients
- S24 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from mammalian cells and tissues by sedimentation in discontinuous gradients
- S25 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from cells and tissues in sedimentation velocity gradients
- S26 – Isolation of plasma membrane from cardiac muscle
- S27 – Fractionation of plasma membrane and plasma membrane domains using cationic colloidal silica
- S28 – Fractionation of brush border and basolateral plasma membrane domains from intestinal (ileal) mucosa
- S29 – Fractionation of apical and basolateral plasma membrane domains from Caco-2 cells
- S30 – Fractionation of plasma membrane microdomains of the brush border from renal cortex tissue and from glomeruli (slit diaphragms)
- S31 – Analysis of plasma membrane domains and apical junctional complex from polarized epithelial cells in a self-generated gradient
- S32 – Purification of lipid rafts from cells and tissues (detergent method)
- S33 – Purification of lipid rafts from cells and tissues (detergent-free method)
- S34 – Purification of caveolae from cells and tissues by sonication of a plasma membrane fraction (detergent-free)
- S35 – Separation of membrane-bound and cytosolic proteins (flotation through a discontinuous gradient)
- S36 – Separation of membrane vesicles and cytosol in a self-generated gradient
- S37 – Determination of the cytosolic or membrane location of a large protein complex
- S38 – Isolation of secretory granules from pancreatic cells
- S39 – Isolation of a granule fraction from eosinophils
- S40a – Preparation of synaptosomes, synaptoneurosomes, neuromelanin granules and synaptic vesicles – a methodological survey
- S40 – Isolation of vesicular and granular fractions from various sources
- S41 – Resolution of smooth endoplasmic reticulum (SER), SER domains, study of SER communication with other organelles and lipid droplets
- S42 – Endocytosis analysis – a review of density gradient methods
- S43 – Lysosomes – a methodological review
- S44 – Endocytosis of ligands: analysis in sedimentation velocity gradients
- S45 – Endocytosis of ligands: fractionation of clathrin-coated vesicles, endosomes and lysosomes by buoyant density in a self-generated gradient
- S46 – Endocytosis in cultured cells: analysis of endosomes, lysosomes and plasma membrane by buoyant density
- S47a – Intracellular exocytic vesicle trafficking and exocyst complex – a short methodological summary
- S47 – Analysis of exocytosis, exocyst function and plasma membrane domain targeting in (A) self-generated and (B) pre-formed gradients
- S48 – Analysis of membrane trafficking and intracellular signaling in selfgenerated gradients
- S49 – Analysis of membranes from Drosophila
- S50 – Fractionation of acidocalcisomes from trypanosomes (and other protozoa) and Gram-negative bacteria
- S51 – Isolation of optical system membranes and structures
- S52 – Fractionation of vacuoles, pre-vacuoles, vacuolar, subvacuolar vesicles, secretory vesicles and Cvt vesicles from yeast spheroplasts
- S53 – Fractionation of ER, Golgi, TGN, endosomes and vacuoles from yeast spheroplasts
- S54 – Endocytosis in rat liver: analysis of lysosome and late endosome events using a two-phase Nycodenz®-polysucrose gradient
- S55 – Isolation of mammalian lysosomes in discontinuous gradients
- S56 – Analysis of mammalian lysosomes (ER, endosomes and plasma membrane) in continuous gradients
- S57 – Isolation of peroxisomes from yeast spheroplasts
- S58 – Isolation of organelles from protozoa and amoeba
- S59 – Isolation of organelles from algae and fungi
- S60 – Isolation of organelles from plants and plant cells
- S61 – Membrane trafficking and proteomic analysis of plants and plant cells
- S62 – Extracellular vesicles from non-mammalian sources
- S63 – Mammalian cell exosomes and other microvesicles from conditioned medium
- S64 – Isolation of plasma membrane from cultured cells by flotation through a discontinuous gradient
- Subcellular-indexes-Serumwerk
Subcellular References Serumwerk
- RS01 – Purification of nuclei from tissues and cells
- RS02 – Purification and analysis of peroxisomes
- RS03 – Purification of mitochondria from mammalian sources
- RS04 – Purification of and analysis of lysosomes
- RS05 – Analysis of membrane trafficking in mammalian tissues and cells: fractionation of ER, Golgi, TGN, PM and endosomes
- RS06 – Lipid rich detergent-resistant domains (mammalian sources)
- RS07 – Detergent-free strategy for lipid raft isolation from mammalian cells, tissues and organelles
- RS08 – Purification of caveolae in gradients prepared from OptiPrep™
- RS09 – Lipid-rich membranes from non-mammalian sources
- RS10 – Mammalian (including all chordata) cell exosomes and other microvesicles from, tissues, cells and conditioned medium
- RS11 – Extracellular vesicles from non-mammalian sources
- RS12 – Endocytosis – studies on various tissue and cell types
- RS13 – Resolution of soluble cytosolic proteins from membrane vesicles and organelles
- RS14 – Purification of organelles and membranes from non-mammalian eukaryotes
- RS15 – Purification of subcelluar organelles and membrane compartments from Saccharomyces cerevisiae – a bibliography
Virus Applications Serumwerk
- V01 – Preparation of density gradient solutions
- V02 – Preparation of discontinuous and continuous gradients
- V03 – Preparation of self-generating gradients
- V04 – Harvesting gradients
- V05 – Analysis of gradients
- V06 – Purification of viruses and viral vectors using OptiPrep™
- V07 – Purification of Group I (ds)DNA viruses: adenovirus and removal of helper virus
- V08 – Purification of Group I (ds)DNA viruses:
Herpesviridae and Baculoviridae in a self-generated gradient - V09 – Purification of Group I (ds)DNA viruses:
Herpesviridae and Asfaviridae in a pre-formed gradient - V10 – Purification of Group I (ds)DNA viruses: human papillomavirus
- V11 – Purification of Group I (ds)DNA viruses: Polyomaviridae
- V12 – Purification of Group I (ds)DNA viruses: Poxviridae
- V13 – Purification of Group I (ds)DNA viruses (non-mammalian sources): Iridoviridae
- V14 – Purification of Group II (ss)DNA viruses: Parvoviridae: recombinant adenoassociated virus (rAAV) and avian AAV
- V15 – Purification of Group II (ss)DNA viruses: Protoparvovirus – murine minute virus
- V16 – Purification of Group II (ss)DNA viruses: Parvovirinae – parvovirus
- V17 – Purification of Group III (ds)RNA viruses: Reoviridae
- V18 – Purification of Class IV ((+)ssRNA) viruses: Caliciviridae family
- V19 – Purification of Group IV ((+)ss) RNA viruses: Flaviviridae family and hepatitis E in pre-formed gradients
- V20 – Purification of Group IV ((+)ss) RNA viruses: Flaviviridae family: hepatitis C particles in a self-generated gradient
- V21 – Purification of Group IV ((+)ss) RNA viruses: Nidovirales: Coronaviridae and Arteriviridae
- V22 – Purification of Group IV ((+)ss) RNA viruses: Togaviridae: Alphavirus and Rubivirus
- V23 – Purification of Group V ((-)ss) RNA viruses: Arenaviruses: Lassa virus, Tacaribe virus, Junin virus, Lymphocytic choriomeningitic virus (LCV)
- V24 – Purification of Group V ((-)ss) RNA viruses: Bunyaviridae – Hantavirus, Orthobunyavirus and Phlebovirus
- V25 – Purification of Group V ((-)ss) RNA viruses: Ebola virus capsid assembly analysis and purification of Ebola pseudovirus in self-generated gradients
- V26 – Purification of Group V ((-)ss) RNA viruses: Orthomyxoviridae
- V27 – Purification of Group V ((-)ss) RNA viruses: Rhabdoviridae: Rabies virus (Lyssavirus) and vesicular stomatitis virus
- V28 – Purification of Group V ((-)ss) RNA viruses: Paramyxoviridae – Paramyxovirinae and Pneumovirinae
- V29 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Alpharetrovirus: Rous sarcoma virus
- V30 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Betaretrovirus: Mason-Pfizer Monkey virus
- V31 – Purification of Group VI (ss)RNA-RT viruses: Retroviridae: Deltaretrovirus, human T-cell lymphotropic virus (HTLV-1) and human endogenous retrovirus
- V32 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Gammaretrovirus: murine oncornavirus
- V33 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Gammaretrovirus: Moloney murine leukemia virus
- V34 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Lentivirus: Human immunodeficiency virus – 1 (HIV-1) and lentivirus vectors
- V35 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Spumaretrovirinae: Foamy viruses
- V36 – Purification and analysis of Group VII (ds RNA-RT): Hepadnaviridae: Orthohepadnavirus: hepatitis B virus
- V37 – Purification of viruses from non-mammalian cells and tissues
- V38 – Purification and analysis of bacteriophages
- Virus-index-Serumwerk